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KMID : 0380620120440060658
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Korean Journal of Food Science and Technology
2012 Volume.44 No. 6 p.658 ~ p.665
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Analysis and Uncertainty Estimation of Zearalenone in Cereal-Based Products by LC-MS/MS
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Choi Eun-Jung
Kang Sung-Tae
Jung So-Young
Shin Jae-Min
Chang Min-Su
Lee Sang-Me
Kim Jung-Hun
Chae Young-Zoo
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Abstract
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A survey of zearalenone contamination was conducted on cereal-based products by using an immunoaffinity column with LC-MS/MS. The calibration curve showed good lineality, with correlation coefficients (R2) of 0.999 in the concentration range from 1 to 250 ng/mL. The limits of detection and quantification were approximately 0.3 μg/kg and 1.0 μg/kg, respectively. The recoveries in the barley tea, Misutgaru and snack ranged from 73.6-107.8%. Zearalenone was detected in 10 samples (11.2% incidence). The highest zearalenone contamination level was 29.7 μg/kg in the Misutgaru. This survey was conducted with uncertainty of measurement. The expanded uncertainty for zearalenone was estimated to be 44.9±5.0 μg/kg (k=2, 95% confidence level) and 128.7±7.9 μg/kg (k=2, 95% confidence level) for barley tea, 30.7±5.8 μg/kg (k=2, 95% confidence level) and 173.7±14.9 μg/kg (k=2.26, 95% confidence level) for Misutgaru, and 37.2±7.4 μg/ kg (k=2.31, 95% confidence level) and 151.0±10.4 μg/kg (k=2, 95% confidence level) snack at the level of 41.7 μg/kg and 166.7 μg/kg, respectively.
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KEYWORD
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zearalenone, LC-MS/MS, immunoaffinity column, uncertainty
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